Dog SPA2(Surfactant Associated Protein A2) ELISA Kit

Dog SPA2(Surfactant Associated Protein A2) ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring SPA2 in Tissue homogenates, Cell lysates, Cell culture supernatant, Other biological fluids samples from Dog. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog SPA2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog SPA2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog SPA2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog SPA2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Target background

Alternative names include SFTPA2B; COLEC5; SP2A; SPA1 beta; SP-A2; SPAII; Collectin-5; Pulmonary Surfactant Protein A2; 35 kDa pulmonary surfactant-associated protein; Alveolar proteinosis protein.