Dog HbA1c(Glycated Hemoglobin A1c) ELISA Kit
Dog HbA1c(Glycated Hemoglobin A1c) ELISA Kit from ELK Biotechnology Read more ›
Product Description
Dog HbA1c(Glycated Hemoglobin A1c) ELISA Kit is an ELK Biotechnology ELISA kit for research measurement of HbA1c.
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Dog HbA1c. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog HbA1c. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog HbA1c in the samples is then determined by comparing the OD of the samples to the standard curve.
Target background
Alternative names include Glycosylated Hemoglobin; Hemoglobin A1c; Hb1c; HbAIc; HbAIc.
Key Facts
| Target | HbA1c |
| Also known as | Glycosylated Hemoglobin; Hemoglobin A1c; Hb1c; HbAIc; HbAIc |
| Species reactivity | Dog |
| Form / buffer | Pre-coated microplate kit |
| Research area | Diagnosis of diabetes mellitus |
| Assay type | Competitive Inhibition |
| Detection method | Colorimetric (TMB/HRP) |
| Sensitivity | 4.07 µg/mL |
| Detection range | 15.63-1000 µg/mL |
| Sample types |
PlasmaErythrocyte lysates
See sample validation ›
|
| Plate format | 96-well pre-coated |
Sample Type Validation
1 sample type| Sample Type | SERUM | PLASMA (EDTA) | URINE | CSF | CELL SUPERNATANT |
|---|---|---|---|---|---|
| Sample compatibility |
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.