Collaborator ELK Biotechnology
ELISA KitsISO 9001:2015
ABM83519

Dog HbA1c(Glycated Hemoglobin A1c) ELISA Kit

ISO 9001|ABMIUM Verified™|ELISA

Dog HbA1c(Glycated Hemoglobin A1c) ELISA Kit from ELK Biotechnology Read more ›

Dog HbA1c(Glycated Hemoglobin A1c) ELISA Kit
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Dog HbA1c(Glycated Hemoglobin A1c) ELISA Kit

Product Description

Dog HbA1c(Glycated Hemoglobin A1c) ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring HbA1c in Plasma, Erythrocyte lysates samples from Dog. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Dog HbA1c. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog HbA1c. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog HbA1c in the samples is then determined by comparing the OD of the samples to the standard curve.

Target background

Alternative names include Glycosylated Hemoglobin; Hemoglobin A1c; Hb1c; HbAIc; HbAIc.

Key Facts

Product typeELISA Kits Target: HbA1c
Collaborator ELK Biotechnology
Partner model ELK9768
Target HbA1c
Research areasDiagnosis of diabetes mellitus
Reactivity Dog
Applications ELISA
Assay type Competitive Inhibition
Sample types Plasma,Erythrocyte lysates
Detection range 15.63-1000 µg/mL
Sensitivity 4.07 µg/mL
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Sample Type Validation

1 sample type
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
Sample TypeSERUMPLASMA (EDTA)URINECSFCELL SUPERNATANT
Sample compatibility

Specifications

Target
HbA1c
Also known as
Glycosylated Hemoglobin; Hemoglobin A1c; Hb1c; HbAIc; HbAIc
Form / buffer
Pre-coated microplate kit
Assay type
Competitive Inhibition
Detection method
Colorimetric (TMB/HRP)
Sensitivity
4.07 µg/mL
Detection range
15.63-1000 µg/mL
Sample types
PlasmaErythrocyte lysates
Plate format
96-well pre-coated
Species reactivity
Dog

Storage & Stability

Long-term storage
Store according to the conditions stated in the product manual.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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