Rat RPS6Kb1(Ribosomal Protein S6 Kinase Beta 1) ELISA Kit

Rat RPS6Kb1(Ribosomal Protein S6 Kinase Beta 1) ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring RPS6Kb1 in Tissue homogenates, Other biological fluids samples from Rat. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat RPS6Kb1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat RPS6Kb1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat RPS6Kb1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat RPS6Kb1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Target background

Alternative names include PS6K; S6K; S6K1; STK14A; p70(S6K)-alpha; p70-S6K; p70-alpha; P70-S6 Kinase 1; 70 kDa ribosomal protein S6 kinase 1; Serine/threonine-protein kinase 14A.