Collaborator ELK Biotechnology
ELISA KitsISO 9001:2015
ABM83159

Sheep KISS1(Kisspeptin 1) ELISA Kit

ISO 9001|ABMIUM Verified™|ELISA

Sheep KISS1(Kisspeptin 1) ELISA Kit from ELK Biotechnology Read more ›

Sheep KISS1(Kisspeptin 1) ELISA Kit
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Sheep KISS1(Kisspeptin 1) ELISA Kit

Product Description

Sheep KISS1(Kisspeptin 1) ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring KISS1 in Serum, Plasma, Other biological fluids samples from Sheep. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sheep KISS1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep KISS1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sheep KISS1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep KISS1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Target background

Alternative names include Metastin; KiSS-1 Metastasis-Suppressor.

Key Facts

Product typeELISA Kits Target: KISS1
Collaborator ELK Biotechnology
Partner model ELK9649
Target KISS1
Research areasTumor immunity
Reactivity Sheep
Applications ELISA
Assay type Sandwich
Sample types Serum,Plasma,Other biological fluids
Detection range 15.63-1000 pg/mL
Sensitivity 5.52 pg/mL
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Sample Type Validation

1 sample type
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
Sample TypeSERUMPLASMA (EDTA)URINECSFCELL SUPERNATANT
Sample compatibility

Specifications

Target
KISS1
Also known as
Metastin; KiSS-1 Metastasis-Suppressor
Form / buffer
Pre-coated microplate kit
Assay type
Sandwich
Detection method
Colorimetric (TMB/HRP)
Sensitivity
5.52 pg/mL
Detection range
15.63-1000 pg/mL
Sample types
SerumPlasmaOther biological fluids
Plate format
96-well pre-coated
Species reactivity
Sheep

Storage & Stability

Long-term storage
Store according to the conditions stated in the product manual.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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