Collaborator ELK Biotechnology
ELISA KitsISO 9001:2015
ABM85022

Simian OC/BGP(Osteocalcin) ELISA Kit

ISO 9001|ABMIUM Verified™|ELISA

Simian OC/BGP(Osteocalcin) ELISA Kit from ELK Biotechnology Read more ›

Simian OC/BGP(Osteocalcin) ELISA Kit 96T*5
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Simian OC/BGP(Osteocalcin) ELISA Kit 96T*5

Product Description

Simian OC/BGP(Osteocalcin) ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring Simian OC/BGP in Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernatant, Other biological fluids samples from Simian. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian OC/BGP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian OC/BGP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian OC/BGP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian OC/BGP in the samples is then determined by comparing the OD of the samples to the standard curve.

Target background

Alternative names include BGLAP; BGP; OC; OCN; bone gamma-carboxyglutamate protein; Osteocalcin.

Key Facts

Product typeELISA Kits Target: Simian OC/BGP
Collaborator ELK Biotechnology
Partner model ELK9627
Target Simian OC/BGP
Research areasStem Cells, Signal Transduction
Reactivity Simian
Applications ELISA
Assay type Sandwich
Sample types Serum,Plasma,Tissue homogenates,Cell lysates,Cell culture supernatant,Other biological fluids
Detection range 0.78-50 ng/mL
Sensitivity 0.46 ng/mL
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Sample Type Validation

1 sample type
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
Sample TypeSERUMPLASMA (EDTA)URINECSFCELL SUPERNATANT
Sample compatibility

Specifications

Target
Simian OC/BGP
Also known as
BGLAP; BGP; OC; OCN; bone gamma-carboxyglutamate protein; Osteocalcin
Form / buffer
Pre-coated microplate kit
Assay type
Sandwich
Detection method
Colorimetric (TMB/HRP)
Sensitivity
0.46 ng/mL
Detection range
0.78-50 ng/mL
Sample types
SerumPlasmaTissue homogenatesCell lysatesCell culture supernatantOther biological fluids
Plate format
96-well pre-coated
Species reactivity
Simian

Storage & Stability

Long-term storage
Store according to the conditions stated in the product manual.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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