Mouse BCKDHB(2-oxoisovalerate dehydrogenase subunit beta, mitochondrial) ELISA Kit

Mouse BCKDHB(2-oxoisovalerate dehydrogenase subunit beta, mitochondrial) ELISA Kit is an ELK Biotechnology ELISA kit for research measurement of BCKDHB.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse BCKDHB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse BCKDHB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse BCKDHB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse BCKDHB in the samples is then determined by comparing the OD of the samples to the standard curve.

Target background

Alternative names include 2-oxoisovalerate dehydrogenase subunit beta, mitochondrial; Branched-chain alpha-keto acid dehydrogenase E1 component beta chain; BCKDE1B; BCKDH E1-beta; Bckdhb.