Rat TNFRSF25(Tumor necrosis factor receptor superfamily member 25) ELISA Kit

Rat TNFRSF25(Tumor necrosis factor receptor superfamily member 25) ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring TNFRSF25 in Serum, Plasma, Tissue homogenates, Cell lysates, Other biological fluids samples from Rat. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TNFRSF25. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TNFRSF25. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TNFRSF25, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TNFRSF25 in the samples is then determined by comparing the OD of the samples to the standard curve.

Target background

Alternative names include TNFRSF25; APO3; DDR3; DR3; TNFRSF12; WSL; WSL1; Tumor necrosis factor receptor superfamily member 25; Apo-3; Apoptosis-inducing receptor AIR;Apoptosis-mediating receptor DR3; Apoptosis-mediating receptor TRAMP; Death receptor 3; Lymphocyte-associated receptor of death; LARD; Protein WSL; Protein WSL-1.