Zebrafish HSP-70/HSPA9(HeatShock Protein 70) ELISA Kit

Zebrafish HSP-70/HSPA9(HeatShock Protein 70) ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring HSP-70/HSPA9 in Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernatant, Other biological fluids samples from Zebrafish. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Zebrafish HSP-70/HSPA9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Zebrafish HSP-70/HSPA9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Zebrafish HSP-70/HSPA9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Zebrafish HSP-70/HSPA9 in the samples is then determined by comparing the OD of the samples to the standard curve.

Target background

Alternative names include DnaK; 70 Kilodalton Heat Shock Proteins;Heat shock 70 kDa protein 1; 2; heat shock 70kDa protein 1A; HSP70; HSP70 1; HSP70 1; HSP70 2; HSP70 1A; HSP70.1; HSP70.2; HSP70I; HSP72; HSPA1; HSPA1A; HSPA1B; HSP70; Heat Shock Protein 70.