Rat KEAP1(Kelch Like ECH Associated Protein 1) ELISA Kit

Rat KEAP1(Kelch Like ECH Associated Protein 1) ELISA Kit is an ELK Biotechnology ELISA kit for research measurement of KEAP1.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat KEAP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat KEAP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat KEAP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat KEAP1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Target background

Alternative names include INrf2; KLHL19; Cytosolic inhibitor of Nrf2; Kelch-like protein 19.