Human EGR2(Early Growth Response Protein 2) ELISA Kit

Human EGR2(Early Growth Response Protein 2) ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring EGR2 in Tissue homogenates, Cell lysates, Other biological fluids samples from Human. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EGR2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EGR2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human EGR2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EGR2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Target background

Alternative names include KROX20; Early growth response 2; KROX-20, Drosophila, homolog (early growth response-2) ; CMT1D; AT591.