Rat MHCII(Major histocompatibility complex class II) ELISA Kit

Rat MHCII(Major histocompatibility complex class II) ELISA Kit is an ELK Biotechnology ELISA kit for research measurement of MHCII.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MHCII. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MHCII. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MHCII, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MHCII in the samples is then determined by comparing the OD of the samples to the standard curve.

Target background

Alternative names include Major histocompatibility complex class II; MHCII; HLA-DR; MHC cell surface glycoprotein; MHC class II antigen DRA; MHC II.