ACTb(Actin Beta) ELISA Kit
ACTb(Actin Beta) ELISA Kit from ELK Biotechnology Read more ›
Product Description
ACTb(Actin Beta) ELISA Kit is an ELK Biotechnology ELISA kit for research measurement of ACTb.
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with ACTb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to ACTb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ACTb in the samples is then determined by comparing the OD of the samples to the standard curve.
Target background
Alternative names include ACT-B; PS1TP5BP1; Actin, cytoplasmic 1; Actin, cytoplasmic 1, N-terminally processed.
Key Facts
| Target | ACTb |
| Also known as | ACT-B; PS1TP5BP1; Actin, cytoplasmic 1; Actin, cytoplasmic 1, N-terminally processed |
| Species reactivity | General |
| Form / buffer | Pre-coated microplate kit |
| Research area | Signal transduction, Developmental science, Bone metabolism |
| Assay type | Competitive Inhibition |
| Detection method | Colorimetric (TMB/HRP) |
| Sensitivity | 0.057 ng/mL |
| Detection range | 0.16-10 ng/mL |
| Sample types |
Cell lysatesCell culture supernatantsOther biological fluids
See sample validation ›
|
| Plate format | 96-well pre-coated |
Sample Type Validation
1 sample type| Sample Type | SERUM | PLASMA (EDTA) | URINE | CSF | CELL SUPERNATANT |
|---|---|---|---|---|---|
| Sample compatibility |
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.