Collaborator ELK Biotechnology
ELISA KitsISO 9001:2015
ABM80318

Mouse HCy(Homocysteine) ELISA Kit

ISO 9001|ABMIUM Verified™|ELISA

Mouse HCy(Homocysteine) ELISA Kit from ELK Biotechnology Read more ›

Mouse HCy(Homocysteine) ELISA Kit
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Mouse HCy(Homocysteine) ELISA Kit

Product Description

Mouse HCy(Homocysteine) ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring HCy in Serum, Plasma, Other biological fluids samples from Mouse. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse HCy. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse HCy. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse HCy in the samples is then determined by comparing the OD of the samples to the standard curve.

Target background

Alternative names include Homocysteine.

Key Facts

Product typeELISA Kits Target: HCy
Collaborator ELK Biotechnology
Partner model ELK8718
Target HCy
Research areasatherosclerosis
Reactivity Mouse
Applications ELISA
Assay type Competitive Inhibition
Sample types Serum,Plasma,Other biological fluids
Detection range 125-8000 ng/mL
Sensitivity 38.44 ng/mL
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Sample Type Validation

1 sample type
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
Sample TypeSERUMPLASMA (EDTA)URINECSFCELL SUPERNATANT
Sample compatibility

Specifications

Target
HCy
Also known as
Homocysteine
Form / buffer
Pre-coated microplate kit
Assay type
Competitive Inhibition
Detection method
Colorimetric (TMB/HRP)
Sensitivity
38.44 ng/mL
Detection range
125-8000 ng/mL
Sample types
SerumPlasmaOther biological fluids
Plate format
96-well pre-coated
Species reactivity
Mouse

Storage & Stability

Long-term storage
Store according to the conditions stated in the product manual.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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