Collaborator ELK Biotechnology
ELISA KitsISO 9001:2015
ABM79992

Human CA 27-29(cancer antigen 27-29) ELISA Kit

ISO 9001|ABMIUM Verified™|ELISA

Human CA 27-29(cancer antigen 27-29) ELISA Kit from ELK Biotechnology Read more ›

Human CA 27-29(cancer antigen 27-29) ELISA Kit
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Human CA 27-29(cancer antigen 27-29) ELISA Kit

Product Description

Human CA 27-29(cancer antigen 27-29) ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring CA 27-29 in Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernatant, Other biological fluids samples from Human. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CA 27-29. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CA 27-29. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CA 27-29, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CA 27-29 in the samples is then determined by comparing the OD of the samples to the standard curve.

Target background

Alternative names include CA 27-29.

Key Facts

Product typeELISA Kits Target: CA 27-29
Collaborator ELK Biotechnology
Partner model ELK8602
Target CA 27-29
Research areasCancer
Reactivity Human
Applications ELISA
Assay type Sandwich
Sample types Serum,Plasma,Tissue homogenates,Cell lysates,Cell culture supernatant,Other biological fluids
Detection range 1.57-100 U/mL
Sensitivity 0.8 U/mL
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Sample Type Validation

1 sample type
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
Sample TypeSERUMPLASMA (EDTA)URINECSFCELL SUPERNATANT
Sample compatibility

Specifications

Target
CA 27-29
Also known as
CA 27-29
Form / buffer
Pre-coated microplate kit
Assay type
Sandwich
Detection method
Colorimetric (TMB/HRP)
Sensitivity
0.8 U/mL
Detection range
1.57-100 U/mL
Sample types
SerumPlasmaTissue homogenatesCell lysatesCell culture supernatantOther biological fluids
Plate format
96-well pre-coated
Species reactivity
Human

Storage & Stability

Long-term storage
Store according to the conditions stated in the product manual.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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