Rat pro interleukin 1 beta (pro-IL1b) ELISA Kit
Rat pro interleukin 1 beta (pro-IL1b) ELISA Kit from ELK Biotechnology Read more ›
Product Description
Rat pro interleukin 1 beta (pro-IL1b) ELISA Kit is an ELK Biotechnology ELISA kit for research measurement of pro interleukin 1 beta.
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat pro-IL1b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat pro-IL1b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat pro-IL1b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat pro-IL1b in the samples is then determined by comparing the OD of the samples to the standard curve.
Target background
Alternative names include pro-IL1-B; pro-IL1-Beta; pro-IL1F2; pro-IL-1β.
Key Facts
| Target | pro interleukin 1 beta |
| Also known as | pro-IL1-B; pro-IL1-Beta; pro-IL1F2; pro-IL-1β |
| Species reactivity | Rat |
| Form / buffer | Pre-coated microplate kit |
| Research area | Cytokine, Tumor immunity, Infection immunity |
| Assay type | Sandwich |
| Detection method | Colorimetric (TMB/HRP) |
| Sensitivity | 17.2 pg/mL |
| Detection range | 62.5-4000 pg/mL |
| Sample types |
SerumPlasmaTissue homogenatesCell lysatesOther biological fluids
See sample validation ›
|
| Plate format | 96-well pre-coated |
Sample Type Validation
1 sample type| Sample Type | SERUM | PLASMA (EDTA) | URINE | CSF | CELL SUPERNATANT |
|---|---|---|---|---|---|
| Sample compatibility |
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.