Rat TPO-Ab(anti-Thyroid-Peroxidase antibody) ELISA Kit from ELK Biotechnology Read more ›
Rat TPO-Ab(anti-Thyroid-Peroxidase antibody) ELISA Kit is a research-use antibody from ELK Biotechnology for studying TPO-Ab in ELISA workflows with listed reactivity for Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat TPO-Ab. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TPO-Ab. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TPO-Ab in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative names include TPO Ab; TPO-Ab.
| Collaborator | ELK Biotechnology |
| Partner model | ELK8352 |
| Target | TPO-Ab |
| Research areas | Signal transduction, Metabolic pathway, Infection immunity, Hormone metabolism, Dermatology |
| Reactivity | Rat |
| Applications | ELISA |
| Assay type | Competitive Inhibition |
| Sample types | Serum,Plasma,Cell lysates,Cell culture supernatant,Other biological fluids |
| Detection range | 1.25-80 IU/mL |
| Sensitivity | 1.25 IU/mL |
| Sample Type | SERUM | PLASMA (EDTA) | URINE | CSF | CELL SUPERNATANT |
|---|---|---|---|---|---|
| Sample compatibility |
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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