Horse COL3(Collagen Type III) ELISA Kit

Horse COL3(Collagen Type III) ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring Horse COL3 in Serum, Plasma, Cell lysates, Cell culture supernatant, Other biological fluids samples from Horse. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Horse COL3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse COL3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse COL3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Target background

Alternative names include Collagen Type 3; Col 3; Collagen Type III; Col III.