IP3(Inositol Triphosphate) ELISA Kit from ELK Biotechnology Read more ›
IP3(Inositol Triphosphate) ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring IP3 in Serum, Plasma, Cell lysates, Cell culture supernatant, Other biological fluids samples from General. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with IP3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to IP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of IP3 in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative names include InsP3; Inositol 1,4,5-Trisphosphate; Triphosphoinositol.
| Collaborator | ELK Biotechnology |
| Partner model | ELK8148 |
| Target | IP3 |
| Research areas | Signal transduction, Metabolic pathway, Endocrinology, Cardiovascular biology, Neuro science, Hormone metabolism, Bone metabolism |
| Reactivity | General |
| Applications | ELISA |
| Assay type | Competitive Inhibition |
| Sample types | Serum,Plasma,Cell lysates,Cell culture supernatant,Other biological fluids |
| Detection range | 156.25-10000 pg/mL |
| Sensitivity | 48.6 pg/mL |
| Sample Type | SERUM | PLASMA (EDTA) | URINE | CSF | CELL SUPERNATANT |
|---|---|---|---|---|---|
| Sample compatibility |
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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