cAMP(Cyclic Adenosine Monophosphate) ELISA Kit from ELK Biotechnology Read more ›
cAMP(Cyclic Adenosine Monophosphate) ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring cAMP in Serum, Plasma, Cell lysates, Cell culture supernatant, Other biological fluids samples from General. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with cAMP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to cAMP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of cAMP in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative names include c-AMP; 3'-5'-Cyclic Adenosine Monophosphate; Adenosine Cyclophosphate.
| Collaborator | ELK Biotechnology |
| Partner model | ELK8116 |
| Target | cAMP |
| Research areas | Signal transduction, Metabolic pathway |
| Reactivity | General |
| Applications | ELISA |
| Assay type | Competitive Inhibition |
| Sample types | Serum,Plasma,Cell lysates,Cell culture supernatant,Other biological fluids |
| Detection range | 312.5-20000 pg/mL |
| Sensitivity | 92.2 pg/mL |
| Sample Type | SERUM | PLASMA (EDTA) | URINE | CSF | CELL SUPERNATANT |
|---|---|---|---|---|---|
| Sample compatibility |
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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