Pig FABP3(Fatty Acid Binding Protein 3, Muscle And Heart) ELISA Kit

Pig FABP3(Fatty Acid Binding Protein 3, Muscle And Heart) ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring FABP3 in Serum, Plasma, Other biological fluids samples from Pig. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig FABP3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig FABP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig FABP3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig FABP3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Target background

Alternative names include MDGI; FABP11; H-FABP; O-FABP; Hfabp; Mammary Derived Growth Inhibitor; Fatty Acid Binding Protein 11; Muscle fatty acid-binding protein; Heart-type fatty acid-binding protein.