Dog HMGA1(High Mobility Group AT Hook Protein 1) ELISA Kit

Dog HMGA1(High Mobility Group AT Hook Protein 1) ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring HMGA1 in Tissue homogenates, Other biological fluids samples from Dog. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog HMGA1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog HMGA1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog HMGA1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog HMGA1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Target background

Alternative names include HMG-R; HMGIY; High mobility group protein A1; High mobility group protein R; High mobility group protein HMG-I/HMG-Y.