Collaborator ELK Biotechnology
ELISA KitsISO 9001|ABMIUM Verified™
ABM78341

Sheep GHRL(Ghrelin) ELISA Kit

ISO 9001|ABMIUM Verified™|ELISA

Sheep GHRL(Ghrelin) ELISA Kit from ELK Biotechnology Read more ›

Sheep GHRL(Ghrelin) ELISA Kit
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Sheep GHRL(Ghrelin) ELISA Kit

Product Description

Sheep GHRL(Ghrelin) ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring GHRL in Serum, Plasma, Cell lysates, Saliva, Cell culture supernatant, Other biological fluids samples from Sheep. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Sheep GHRL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep GHRL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep GHRL in the samples is then determined by comparing the OD of the samples to the standard curve.

Target background

Alternative names include MTLRP; Growth Hormone-Releasing Peptide.

Key Facts

Product typeELISA Kits Target: GHRL
Collaborator ELK Biotechnology
Partner model ELK7969
Target GHRL
Research areasSignal transduction, Metabolic pathway, Endocrinology, Gastroenterology, Hormone metabolism
Reactivity Sheep
Applications ELISA
Assay type Competitive Inhibition
Sample types Serum,Plasma,Cell lysates,Saliva,Cell culture supernatant,Other biological fluids
Detection range 156.25-10000 pg/mL
Sensitivity 49.7 pg/mL
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Sample Type Validation

1 sample type
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
Sample TypeSERUMPLASMA (EDTA)URINECSFCELL SUPERNATANT
Sample compatibility

Specifications

Target
GHRL
Also known as
MTLRP; Growth Hormone-Releasing Peptide
Form / buffer
Pre-coated microplate kit
Assay type
Competitive Inhibition
Detection method
Colorimetric (TMB/HRP)
Sensitivity
49.7 pg/mL
Detection range
156.25-10000 pg/mL
Sample types
SerumPlasmaCell lysatesSalivaCell culture supernatantOther biological fluids
Plate format
96-well pre-coated
Species reactivity
Sheep

Storage & Stability

Long-term storage
Store according to the conditions stated in the product manual.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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