Mouse LIPA(Lipase A, Lysosomal Acid) ELISA Kit

Mouse LIPA(Lipase A, Lysosomal Acid) ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring LIPA in Serum, Plasma, Tissue homogenates, Other biological fluids samples from Mouse. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse LIPA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse LIPA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse LIPA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse LIPA in the samples is then determined by comparing the OD of the samples to the standard curve.

Target background

Alternative names include LAL; CESD; Lipase A,Lysosomal Acid,Cholesterol Esterase(Wolman Disease); Acid Cholesteryl Ester Hydrolase; Lysosomal acid lipase/cholesteryl ester hydrolase.