Guinea pig IL-12p40(Interleukin 12 p40) ELISA Kit from ELK Biotechnology Read more ›
Guinea pig IL-12p40(Interleukin 12 p40) ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring IL-12p40 in Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernatant, Other biological fluids samples from Guinea pig. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Guinea pig IL-12p40. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Guinea pig IL-12p40. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Guinea pig IL-12p40, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Guinea pig IL-12p40 in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative names include IL-12B; p40; IL12B; CLMF2; NKSF2; Natural Killer Cell Stimulatory Factor 2; Cytotoxic Lymphocyte Maturation Factor 2; Interleukin 23, P40 Subunit.
| Collaborator | ELK Biotechnology |
| Partner model | ELK5872 |
| Target | IL-12p40 |
| Research areas | Cytokine, Infection immunity, Immune molecule |
| Reactivity | Guinea pig |
| Applications | ELISA |
| Assay type | Sandwich |
| Sample types | Serum,Plasma,Tissue homogenates,Cell lysates,Cell culture supernatant,Other biological fluids |
| Detection range | 7.82-500 pg/mL |
| Sensitivity | 3 pg/mL |
| Sample Type | SERUM | PLASMA (EDTA) | URINE | CSF | CELL SUPERNATANT | |
|---|---|---|---|---|---|---|
| Sample compatibility |
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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