Human ProNT/NMN(Proneurotensin/neuromedin N) ELISA Kit
Human ProNT/NMN(Proneurotensin/neuromedin N) ELISA Kit from ELK Biotechnology Read more ›
Product Description
Human ProNT/NMN(Proneurotensin/neuromedin N) ELISA Kit is an ELK Biotechnology ELISA kit for research measurement of ProNT/NMN.
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human ProNT/NMN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ProNT/NMN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ProNT/NMN in the samples is then determined by comparing the OD of the samples to the standard curve.
Target background
Alternative names include Proneurotensin 1-117.
Key Facts
| Target | ProNT/NMN |
| Also known as | Proneurotensin 1-117 |
| Species reactivity | Human |
| Form / buffer | Pre-coated microplate kit |
| Research area | Neuro science |
| Assay type | Competitive Inhibition |
| Detection method | Colorimetric (TMB/HRP) |
| Sensitivity | 5.72 pg/mL |
| Detection range | 15.63-1000 pg/mL |
| Sample types |
SerumPlasmaCell lysatesCell culture supernatantsOther biological fluids
See sample validation ›
|
| Plate format | 96-well pre-coated |
Sample Type Validation
1 sample type| Sample Type | SERUM | PLASMA (EDTA) | URINE | CSF | CELL SUPERNATANT |
|---|---|---|---|---|---|
| Sample compatibility |
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.