Mouse NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit
Mouse NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit from ELK Biotechnology Read more ›
Product Description
Mouse NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit is an ELK Biotechnology ELISA kit for research measurement of NT-ProBNP.
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse NT-ProBNP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NT-ProBNP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse NT-ProBNP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NT-ProBNP in the samples is then determined by comparing the OD of the samples to the standard curve.
Target background
Alternative names include NT-Pro-BNP; ; N-BNP; ANFB_HUMAN; BNP(1-32); BNP(5-29); BNP-32; Gamma-brain natriuretic peptide; nppb.
Key Facts
| Target | NT-ProBNP |
| Also known as | NT-Pro-BNP; ; N-BNP; ANFB_HUMAN; BNP(1-32); BNP(5-29); BNP-32; Gamma-brain natriuretic peptide; nppb |
| Species reactivity | Mouse |
| Form / buffer | Pre-coated microplate kit |
| Research area | Endocrinology, Cardiovascular biology |
| Assay type | Sandwich |
| Detection method | Colorimetric (TMB/HRP) |
| Sensitivity | 28 pg/mL |
| Detection range | 78.13-5000 pg/mL |
| Sample types |
SerumPlasmaTissue homogenatesCell lysatesCell culture supernatantsOther biological fluids
See sample validation ›
|
| Plate format | 96-well pre-coated |
Sample Type Validation
1 sample type| Sample Type | SERUM | PLASMA (EDTA) | URINE | CSF | CELL SUPERNATANT | |
|---|---|---|---|---|---|---|
| Sample compatibility |
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.