Human UBE2I(Ubiquitin Conjugating Enzyme E2I) ELISA Kit

Human UBE2I(Ubiquitin Conjugating Enzyme E2I) ELISA Kit is an ELK Biotechnology ELISA kit for research measurement of UBE2I.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human UBE2I. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human UBE2I. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human UBE2I, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human UBE2I in the samples is then determined by comparing the OD of the samples to the standard curve.

Target background

Alternative names include P18; UBC9; UBCE9; SUMO-protein ligase; Ubiquitin carrier protein 9; Ubiquitin carrier protein I; Ubiquitin-protein ligase I; SUMO-conjugating enzyme UBC9.