Mouse FceRI(Receptor I For The Fc Region Of Immunoglobulin E) Microsample ELISA Kit

Mouse FceRI(Receptor I For The Fc Region Of Immunoglobulin E) Microsample ELISA Kit is an ELK Biotechnology ELISA kit for research measurement of FceRI.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse FceRI. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse FceRI. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse FceRI, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse FceRI in the samples is then determined by comparing the OD of the samples to the standard curve.

Target background

Alternative names include FCER1A; FCE1A; FcER1 High affinity immunoglobulin epsilon receptor subunit alpha.