Human MEP1b(Meprin A Beta) ELISA Kit

Human MEP1b(Meprin A Beta) ELISA Kit is an ELK Biotechnology ELISA kit for research measurement of MEP1b.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MEP1b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MEP1b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MEP1b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MEP1b in the samples is then determined by comparing the OD of the samples to the standard curve.

Target background

Alternative names include PPH Beta; Endopeptidase-2; Meprin B; PABA Peptide Hydrolase; N-benzoyl-L-tyrosyl-P-amino-Benzoic Acid Hydrolase Subunit Beta.