Human CH25H(Cholesterol-25-Hydroxylase) ELISA Kit

Human CH25H(Cholesterol-25-Hydroxylase) ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring CH25H in Serum, Plasma, Tissue homogenates, Other biological fluids samples from Human. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CH25H. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CH25H. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CH25H, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CH25H in the samples is then determined by comparing the OD of the samples to the standard curve.

Target background

Alternative names include h25OH; Cholesterol 25-monooxygenase.