Human SQSTM1(Sequestosome 1) ELISA Kit

Human SQSTM1(Sequestosome 1) ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring SQSTM1 in Tissue homogenates, Cell lysates, Other biological fluids samples from Human. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SQSTM1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SQSTM1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SQSTM1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SQSTM1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Target background

Alternative names include OSIL; PDB3; ZIP3; p62; p62B; p60; A170; Paget Disease Of Bone 3; Oxidative Stress Induced Like; EBI3-associated protein of 60 kDa; Ubiquitin-binding protein p62.