Rat S100B(S100 Calcium Binding Protein B) Microsample ELISA Kit

Rat S100B(S100 Calcium Binding Protein B) Microsample ELISA Kit is an ELK Biotechnology ELISA kit for research measurement of S100B.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat S100B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat S100B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat S100B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat S100B in the samples is then determined by comparing the OD of the samples to the standard curve.

Target background

Alternative names include S100-B; NEF; S100Beta; S-100 protein subunit beta; S100 protein beta chain; Soluble protein-100B; Soluble protein 100B.