Collaborator ELK Biotechnology
ELISA KitsISO 9001:2015
ABM84558

Simian HBb(Hemoglobin Beta) ELISA Kit

ISO 9001|ABMIUM Verified™|ELISA

Simian HBb(Hemoglobin Beta) ELISA Kit from ELK Biotechnology Read more ›

Simian HBb(Hemoglobin Beta) ELISA Kit
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Simian HBb(Hemoglobin Beta) ELISA Kit

Product Description

Simian HBb(Hemoglobin Beta) ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring Simian HBb in Serum, Plasma, Erythrocyte lysates samples from Simian. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Simian HBb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian HBb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian HBb in the samples is then determined by comparing the OD of the samples to the standard curve.

Target background

Alternative names include HB-B; HBD; CD113t-C; Beta Globin; Spinorphin.

Key Facts

Product typeELISA Kits Target: Simian HBb
Collaborator ELK Biotechnology
Partner model ELK11606
Target Simian HBb
Research areasHematology
Reactivity Simian
Applications ELISA
Assay type Competitive Inhibition
Sample types Serum,Plasma,Erythrocyte lysates
Detection range 7.82-500 pg/mL
Sensitivity 2.82 pg/mL
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Sample Type Validation

1 sample type
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
Sample TypeSERUMPLASMA (EDTA)URINECSFCELL SUPERNATANT
Sample compatibility

Specifications

Target
Simian HBb
Also known as
HB-B; HBD; CD113t-C; Beta Globin; Spinorphin
Form / buffer
Pre-coated microplate kit
Assay type
Competitive Inhibition
Detection method
Colorimetric (TMB/HRP)
Sensitivity
2.82 pg/mL
Detection range
7.82-500 pg/mL
Sample types
SerumPlasmaErythrocyte lysates
Plate format
96-well pre-coated
Species reactivity
Simian

Storage & Stability

Long-term storage
Store according to the conditions stated in the product manual.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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