Simian sCD14(soluble cluster of differentiation 14) ELISA Kit

Simian sCD14(soluble cluster of differentiation 14) ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring Simian sCD14 in Serum, Plasma, Cell lysates, Other biological fluids samples from Simian. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian sCD14. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian sCD14. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian sCD14, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian sCD14 in the samples is then determined by comparing the OD of the samples to the standard curve.

Target background

Alternative names include CD14 Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein; CD antigen CD14; soluble cluster of differentiation 14.