Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM92362

PI3 Kinase P85 Rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

PI3 Kinase P85 Rabbit pAb from ELK Biotechnology Read more ›

PI3 Kinase P85 Rabbit pAb
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PI3 Kinase P85 Rabbit pAb

Product Description

PI3 Kinase P85 Rabbit pAb is a research-use antibody from ELK Biotechnology for studying PI3 Kinase P85 in WB, IHC workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Phosphatidylinositol-4,5-bisphosphate 3-kinase (also called phosphatidylinositide 3-kinases, phosphatidylinositol-3-kinases, PI 3-kinases, PI(3)Ks, PI-3Ks or by the HUGO official stem symbol for the gene family, PI3K(s)) are a family of enzymes involved in cellular functions such as cell growth, proliferation, differentiation, motility, survival and intracellular trafficking, which in turn are involved in cancer.

Additional antibody information

Validation evidence captions Western blot analysis of 1) Hela Cell Lysate, 2) Rat Liver Tissue Lysate , 3) 3T3 Cell Lysate using PI3K p85(EA364)Rabbit pAb diluted at 1:2000. | Immunohistochemical analysis of paraffin-embedded Human Brain Tissue using PI3K p85(EA364)Rabbit pAb diluted at 1:200. | Immunohistochemical analysis of paraffin-embedded Human Pancreas Tissue using PI3K p85(EA364)Rabbit pAb diluted at 1:200.

Key Facts

Product typePrimary Antibody Target: PI3 Kinase P85
Collaborator ELK Biotechnology
Partner model EA364
Target PI3 Kinase P85
Research areasAntibody Research
Reactivity Human,Mouse,Rat
Applications WB,IHC
ConjugationUnconjugated
Dilution rangeWB 1:1,000-2,000 IHC 1:100-200
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Recombinant Protein
Concentration 1mg/mL
Assay type Antibody validation/application
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:1
Mouse
Rat
ABMIUM Validated™ IHC
Species Dilution Notes

Specifications

Target
PI3 Kinase P85
Concentration
1mg/mL
Working concentration
WB 1:1,000-2,000 IHC 1:100-200
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WBIHC
Immunogen
Recombinant Protein
Observed band
85kDa
Cellular localization
Cytosol,Endoplasmic reticulum,Golgi apparatus,Nucleus,Plasma Membrane
Dilution range
WB 1:1,000-2,000 IHC 1:100-200

Storage & Stability

Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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