Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM92349

ATG4c Rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

ATG4c Rabbit pAb from ELK Biotechnology Read more ›

ATG4c Rabbit pAb
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ATG4c Rabbit pAb

Product Description

ATG4c Rabbit pAb is a research-use antibody from ELK Biotechnology for studying ATG4c in IHC workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Control of autophagy was largely discovered in yeast and involves proteins encoded by a set of autophagy-related genes (Atg). Formation of autophagic vesicles requires a pair of essential ubiquitin-like conjugation systems, Atg12-Atg5 and Atg8-phosphatidylethanolamine (Atg8-PE), which are widely conserved in eukaryotes. Numerous mammalian counterparts to yeast Atg proteins have been described, including three Atg8 proteins (GATE-16, GABARAP, and LC3) and four Atg4 homologs (Atg4A/autophagin-2, Atg4B/autophagin-1, Atg4C/autophagin-3, and Atg4D/autophagin-4).

Additional antibody information

Validation evidence captions Immunohistochemical analysis of paraffin-embedded Human Skeletal Muscle Tissue using ATG4c(EA349)Rabbit pAb diluted at 1:200 | Immunohistochemical analysis of paraffin-embedded Human Brain Tissue using ATG4c (EA349)Rabbit pAb diluted at 1:200

Key Facts

Product typePrimary Antibody Target: ATG4c
Collaborator ELK Biotechnology
Partner model EA349
Target ATG4c
Research areasAntibody Research
Reactivity Human,Mouse,Rat
Applications IHC
ConjugationUnconjugated
Dilution rangeIHC 1:100-200
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Recombinant Protein
Concentration 1mg/mL
Assay type Antibody validation/application
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ IHC
Species Dilution Notes
Human IHC 1:100-200
Mouse
Rat

Specifications

Target
ATG4c
Also known as
APG4C, Autl3,Cysteine protease ATG4C antibody
Concentration
1mg/mL
Working concentration
IHC 1:100-200
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
IHC
Immunogen
Recombinant Protein
Observed band
57kDa
Cellular localization
Cytoplasm
Dilution range
IHC 1:100-200

Storage & Stability

Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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