Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM92258

TNF α Rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

TNF α Rabbit pAb from ELK Biotechnology Read more ›

TNF α Rabbit pAb
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TNF α Rabbit pAb

Product Description

TNF α Rabbit pAb is a research-use antibody from ELK Biotechnology for studying TNF α in WB, IHC workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

TNF-α, the prototypical member of the TNF protein superfamily, is a homotrimeric type-II membrane protein. It can cause cytolysis of certain tumor cell lines; it is involved in the induction of cachexia; it is a potent pyrogen, causing fever by direct action or by stimulation of interlerukin-1 secretion; it can stimulate cell proliferation and induce cell differentiation under certain conditions..

Additional antibody information

Validation evidence captions Western blot analysis of LPS Treated HepG2 with TNF α Rabbit pAb EA251 diluted at 1:2,000. | Western blot analysis of Recombinant Human TNF a Protein with TNF α Rabbit pAb EA251 diluted at 1:2,000. | Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma using TNF a(EA251)Rabbit pAb diluted at 1:200.

Key Facts

Product typePrimary Antibody Target: TNF α
Collaborator ELK Biotechnology
Partner model EA251
Target TNF α
Research areasAntibody Research
Reactivity Human,Rat,Mouse
Applications WB,IHC
ConjugationUnconjugated
Dilution rangeWB 1:1,000-2,000 IHC 1:100-200
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Recombinant Protein
Concentration 1mg/mL
Assay type Antibody validation/application
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:1
Rat
Mouse
ABMIUM Validated™ IHC
Species Dilution Notes

Specifications

Target
TNF α
Also known as
APC1 antibody, DIF antibody, TNFSF2 antibody, Tumor necrosis factor alpha antibody
Concentration
1mg/mL
Working concentration
WB 1:1,000-2,000 IHC 1:100-200
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WBIHC
Immunogen
Recombinant Protein
Observed band
17,25kDa
Cellular localization
Secreted,Cell membrane
Dilution range
WB 1:1,000-2,000 IHC 1:100-200

Storage & Stability

Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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