ABM92252
Cyclin B1 Rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
Cyclin B1 Rabbit pAb from ELK Biotechnology Read more ›
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Product Description
Cyclin B1 Rabbit pAb is an ELK Biotechnology antibody for research workflows involving Cyclin B1.
Background
Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34 (Cdk1) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript that is expressed predominantly during G2/M phase of the cell cycle.
Additional antibody information
| Validation evidence captions | Western blot analysis of 1) Jurkat, 2) Hela, 3) C2C12, 4) Rat Liver Tissue with Cyclin B1 Rabbit pAb EA244 diluted at 1:2,000. | Immunohistochemical analysis of paraffin-embedded Human Tonsil Carcinoma using Cyclin B1 (EA244）Rabbit pAb diluted at 1:500. |
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Key Facts
Product type
Primary Antibody›
Target: Cyclin B1
| Target | Cyclin B1 |
| Also known as | CCNB1 antibody, G2 mitotic specific cyclin B1 antibody |
| Concentration | 1mg/mL |
| Working concentration | WB 1:1,000-2,000 IHC 1:200-500 |
| Species reactivity | Human, Rat, Mouse |
| Observed band | 55kDa |
| Cellular localization | Cytoplasm, Cytoskeleton, Nucleus |
| Form / buffer | Antibody solution |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHC |
| Dilution range | WB 1:1,000-2,000 IHC 1:200-500 |
| Immunogen | Recombinant Protein |
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Citations
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:1 | — |
| Rat | — | — |
| Mouse | — | — |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|
Specifications
Target
Cyclin B1
Also known as
CCNB1 antibody, G2 mitotic specific cyclin B1 antibody
Concentration
1mg/mL
Working concentration
WB 1:1,000-2,000 IHC 1:200-500
Form / buffer
Antibody solution
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human, Rat, Mouse
Tested applications
WBIHC
Immunogen
Recombinant Protein
Observed band
55kDa
Cellular localization
Cytoplasm, Cytoskeleton, Nucleus
Dilution range
WB 1:1,000-2,000 IHC 1:200-500
Storage & Stability
Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Storage conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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