Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM92250

Smad2 Rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

Smad2 Rabbit pAb from ELK Biotechnology Read more ›

Smad2 Rabbit pAb
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Smad2 Rabbit pAb

Product Description

Smad2 Rabbit pAb is a research-use antibody from ELK Biotechnology for studying Smad2 in WB, IHC workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

SMAD2 is a protein that in humans is encoded by the SMAD2 gene. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. SMAD2 mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein.

Additional antibody information

Validation evidence captions Western blot analysis of 1) Hela, 2)Jurkat, 3) C2C12, 4) PC12 with Smad2 Rabbit pAb EA241 diluted at 1:2,000. | Immunohistochemical analysis of paraffin-embedded Mouse Heart Tissue using Smad2 (EA241)Rabbit pAb diluted at 1:200.

Key Facts

Product typePrimary Antibody Target: Smad2
Collaborator ELK Biotechnology
Partner model EA241
Target Smad2
Research areasAntibody Research
Reactivity Human,Rat,Mouse
Applications WB,IHC
ConjugationUnconjugated
Dilution rangeWB 1:1,000-2,000 IHC 1:100-200
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Recombinant Protein
Concentration 1mg/mL
Assay type Antibody validation/application
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:1
Rat
Mouse
ABMIUM Validated™ IHC
Species Dilution Notes

Specifications

Target
Smad2
Also known as
hMAD2 antibody, JV18 antibody, MAD antibody, MADH2 antibody, MGC22139 antibody
Concentration
1mg/mL
Working concentration
WB 1:1,000-2,000 IHC 1:100-200
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WBIHC
Immunogen
Recombinant Protein
Observed band
60kDa
Cellular localization
Cytoplasm, Nucleus
Dilution range
WB 1:1,000-2,000 IHC 1:100-200

Storage & Stability

Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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