ABM92241
α-actinin Rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
α-actinin Rabbit pAb from ELK Biotechnology Read more ›
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Product Description
α-actinin Rabbit pAb is an ELK Biotechnology antibody for research workflows involving α-actinin.
Background
α-Actinin belongs to the spectrin family of cytoskeletal proteins. α-Actinin is necessary for the attachment of actin filaments to the Z-lines in skeletal muscle cells, and to the dense bodies in smooth muscle cells. The functional protein is an anti-parallel dimer, which cross-links the thin filaments in adjacent sarcomeres, and therefore coordinated contractions between sarcomeres in the horizontal axis.
Additional antibody information
| Validation evidence captions | Western blot analysis of 1) 3T3, 2)PC12, 3)Rat Heart Tissue with α-actinin Rabbit pAb EA230 diluted at 1:2,000. | Immunohistochemical analysis of paraffin-embedded Mouse KidneyTissue using α-actinin （EA230）Rabbit pAb diluted at 1:500. |
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Key Facts
Product type
Primary Antibody›
Target: α-actinin
| Target | α-actinin |
| Also known as | actinin 1 smooth muscle antibody, actinin alpha 1 antibody, ACTN 1 antibody, FLJ40884 antibody |
| Concentration | 1mg/mL |
| Working concentration | WB 1:1,000-2,000 IHC 1:200-500 |
| Species reactivity | Rat, Mouse |
| Observed band | 103kDa |
| Cellular localization | Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane |
| Form / buffer | Antibody solution |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHC |
| Dilution range | WB 1:1,000-2,000 IHC 1:200-500 |
| Immunogen | Recombinant Protein |
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Citations
ISO 9001
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Reactivity & Application Validation
2 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Rat | |||||||
| Mouse |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Rat | WB 1:1 | — |
| Mouse | — | — |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|
Specifications
Target
α-actinin
Also known as
actinin 1 smooth muscle antibody, actinin alpha 1 antibody, ACTN 1 antibody, FLJ40884 antibody
Concentration
1mg/mL
Working concentration
WB 1:1,000-2,000 IHC 1:200-500
Form / buffer
Antibody solution
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Rat, Mouse
Tested applications
WBIHC
Immunogen
Recombinant Protein
Observed band
103kDa
Cellular localization
Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane
Dilution range
WB 1:1,000-2,000 IHC 1:200-500
Storage & Stability
Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Storage conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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