Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM92230

AMPKα1 Rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

AMPKα1 Rabbit pAb from ELK Biotechnology Read more ›

AMPKα1 Rabbit pAb 50µL/100µL
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AMPKα1 Rabbit pAb 50µL/100µL

Product Description

AMPKα1 Rabbit pAb is a research-use antibody from ELK Biotechnology for studying AMPKα1 in WB, IHC workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

AMP-activated protein kinase (AMPK) is highly conserved from yeast to plants and animals and plays a key role in the regulation of energy homeostasis. AMPK is a heterotrimeric complex composed of a catalytic α subunit and regulatory β and γ subunits, each of which is encoded by two or three distinct genes (α1, 2; β1, 2; γ1, 2, 3).

Additional antibody information

Validation evidence captions Western blot analysis of 1) Hela, 2)3T3, 3)PC12 with AMPK α1 Rabbit pAb EA218 diluted at 1:2,000. | Immunohistochemical analysis of paraffin-embedded Mouse Skeletal Muslce Tissue using AMPK α1 (EA218)Mouse mAb diluted at 1:500.

Key Facts

Product typePrimary Antibody Target: AMPKα1
Collaborator ELK Biotechnology
Partner model EA218
Target AMPKα1
Research areasAntibody Research
Reactivity Human,Rat,Mouse
Applications WB,IHC
ConjugationUnconjugated
Dilution rangeWB 1:500-1,000 IHC 1:200-500
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Recombinant Protein
Concentration 1mg/mL
Assay type Antibody validation/application
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-1
Rat
Mouse
ABMIUM Validated™ IHC
Species Dilution Notes

Specifications

Target
AMPKα1
Also known as
AAPK1, 5 AMP activated protein kinase alpha 1 catalytic subunit, PRKAA1, SNF1A, MGC33776
Concentration
1mg/mL
Working concentration
WB 1:500-1,000 IHC 1:200-500
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WBIHC
Immunogen
Recombinant Protein
Observed band
63kDa
Cellular localization
Cytoplasm, Nucleus
Dilution range
WB 1:500-1,000 IHC 1:200-500

Storage & Stability

Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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