ABM92196
LC3B Rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
LC3B Rabbit pAb from ELK Biotechnology Read more ›
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Product Description
LC3B Rabbit pAb is an ELK Biotechnology antibody for research workflows involving LC3B.
Background
Autophagy marker Light Chain 3 (LC3) was originally identified as a subunit of microtubule-associated proteins 1A and 1B (termed MAP1LC3).
Additional antibody information
| Validation evidence captions | Western blot analysis of 1) Hela, 2) 3T3, 3)Rat Brain Tissue with LC3B Rabbit pAb EA174 diluted at 1:2,000. | Immunohistochemical analysis of paraffin-embedded Rat BrainTissue using LC3B（EA174）Rabbit pAb diluted at 1:500. |
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Key Facts
Product type
Primary Antibody›
Target: LC3B
| Target | LC3B |
| Also known as | ATG8F antibody, MAP1 light chain 3 like protein 2 antibody, MAP1A/MAP1B LC3 B antibody |
| Concentration | 1mg/mL |
| Working concentration | WB 1:1,000-2,000 IHC 1:200-500 |
| Species reactivity | Human, Rat, Mouse |
| Observed band | 14,16kDa |
| Cellular localization | Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Membrane, Microtubule |
| Form / buffer | Antibody solution |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHC |
| Dilution range | WB 1:1,000-2,000 IHC 1:200-500 |
| Immunogen | Recombinant Protein |
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Citations
ISO 9001
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:1 | — |
| Rat | — | — |
| Mouse | — | — |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|
Specifications
Target
LC3B
Also known as
ATG8F antibody, MAP1 light chain 3 like protein 2 antibody, MAP1A/MAP1B LC3 B antibody
Concentration
1mg/mL
Working concentration
WB 1:1,000-2,000 IHC 1:200-500
Form / buffer
Antibody solution
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human, Rat, Mouse
Tested applications
WBIHC
Immunogen
Recombinant Protein
Observed band
14,16kDa
Cellular localization
Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Membrane, Microtubule
Dilution range
WB 1:1,000-2,000 IHC 1:200-500
Storage & Stability
Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Storage conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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