ABM92183
HSP27 Rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
HSP27 Rabbit pAb from ELK Biotechnology Read more ›
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Product Description
HSP27 Rabbit pAb is an ELK Biotechnology antibody for research workflows involving HSP27.
Background
Heat shock protein 27 (Hsp27) also known as heat shock protein beta-1 (HSPB1) is a protein that in humans is encoded by the HSPB1 gene. Hsp27 is a chaperone of the sHsp (small heat shock protein) group among ubiquitin, α-crystallin, Hsp20 and others.Involved in stress resistance and actin organization.
Additional antibody information
| Validation evidence captions | Western blot analysis of 1) Hela, 2)C2C12, 3)Mouse Brain, 4) Rat Brain, with HSP27 Rabbit pAb EA155 diluted at 1:2,000. | Immunohistochemical analysis of paraffin-embedded Mouse Kidney Tissue using HSP27（EA155）Rabbit pAb diluted at 1:500. |
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Key Facts
Product type
Primary Antibody›
Target: HSP27
| Target | HSP27 |
| Also known as | Heat shock protein 27, CMT2F, HSP B1, HSP28, SRP27, Stress responsive protein 27 |
| Concentration | 1mg/mL |
| Working concentration | WB 1:1,000-2,000 IHC 1:200-500 |
| Species reactivity | Human, Rat, Mouse |
| Observed band | 27kDa |
| Cellular localization | Cytoplasm, Cytoskeleton, Nucleus |
| Form / buffer | Antibody solution |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHC |
| Dilution range | WB 1:1,000-2,000 IHC 1:200-500 |
| Immunogen | Recombinant Protein |
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Citations
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:1 | — |
| Rat | — | — |
| Mouse | — | — |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|
Specifications
Target
HSP27
Also known as
Heat shock protein 27, CMT2F, HSP B1, HSP28, SRP27, Stress responsive protein 27
Concentration
1mg/mL
Working concentration
WB 1:1,000-2,000 IHC 1:200-500
Form / buffer
Antibody solution
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human, Rat, Mouse
Tested applications
WBIHC
Immunogen
Recombinant Protein
Observed band
27kDa
Cellular localization
Cytoplasm, Cytoskeleton, Nucleus
Dilution range
WB 1:1,000-2,000 IHC 1:200-500
Storage & Stability
Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Storage conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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