Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM92170

Histone H1(Phospho Thr17) Rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

Histone H1(Phospho Thr17) Rabbit pAb from ELK Biotechnology Read more ›

Histone H1(Phospho Thr17) Rabbit pAb
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Histone H1(Phospho Thr17) Rabbit pAb

Product Description

Histone H1(Phospho Thr17) Rabbit pAb is a research-use antibody from ELK Biotechnology for studying Histone H1(Phospho Thr17) in WB, IHC workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Histone H1 is one of the five main histone protein families which are components of chromatin in eukaryotic cells. May play a key role in the control of gene expression during oogenesis and early embryogenesis, presumably through the perturbation of chromatin structure.

Additional antibody information

Validation evidence captions Western blot analysis of extracts from Hela cells, untreated (-) or treated with Nocodazole (1µg/ml, 24 hr; +), using Phospho-Histone H1(Thr17) Rabbit pAb (upper) EA111 1:2,000 and β-actin Mouse mAb (lower) EM1031 1:5000. | Immunohistochemical analysis of paraffin-embedded Human Breast Caricnoma using Histone H1 (Phospho Thr17)(EA111)Rabbit pAb diluted at 1:500.

Key Facts

Product typePrimary Antibody Target: Histone H1(Phospho Thr17)
Collaborator ELK Biotechnology
Partner model EA111
Target Histone H1(Phospho Thr17)
Research areasAntibody Research
Reactivity Human,Rat,Mouse
Applications WB,IHC
ConjugationUnconjugated
Dilution rangeWB 1:1,000-2,000 IHC 1:200-500
Host Rabbit
Clonality Polyclonal
Isotype Antiserum
Immunogen Synthetic Peptide
Concentration 1mg/mL
Assay type Antibody validation/application
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:1
Rat
Mouse
ABMIUM Validated™ IHC
Species Dilution Notes

Specifications

Target
Histone H1(Phospho Thr17)
Also known as
H1 antibody, HIST1H1B antibody, Histone 1 antibody, Histone H1a antibody
Concentration
1mg/mL
Working concentration
WB 1:1,000-2,000 IHC 1:200-500
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
Antiserum
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WBIHC
Immunogen
Synthetic Peptide
Observed band
17-25kDa
Cellular localization
Cytoplasm,Nucleus,Chromosome
Dilution range
WB 1:1,000-2,000 IHC 1:200-500

Storage & Stability

Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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