Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM92162

Cytokeratin 18 Rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

Cytokeratin 18 Rabbit pAb from ELK Biotechnology Read more ›

Cytokeratin 18 Rabbit pAb
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Cytokeratin 18 Rabbit pAb

Product Description

Cytokeratin 18 Rabbit pAb is a research-use antibody from ELK Biotechnology for studying Cytokeratin 18 in WB, IHC workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Keratin 18 is a type I cytokeratin. It is, together with its filament partner keratin 8, perhaps the most commonly found products of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.Keratin 18 is often used together with keratin 8 and keratin 19 to differentiate cells of epithelial origin from hematopoietic cells in tests that enumerate circulating tumor cells in blood.

Additional antibody information

Validation evidence captions Western blot analysis of Hela with EA056 Rabbit pAb diluted at 1:2,000. | Immunohistochemical analysis of paraffin-embedded Mouse Kidney Tissue using Cytokeratin 18(EA056) Rabbit pAb diluted at 1:500.

Key Facts

Product typePrimary Antibody Target: Cytokeratin 18
Collaborator ELK Biotechnology
Partner model EA056
Target Cytokeratin 18
Research areasAntibody Research
Reactivity Human,Rat,Mouse
Applications WB,IHC
ConjugationUnconjugated
Dilution rangeWB 1:1,000-2,000 IHC 1:500-1,000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Recombinant Protein
Concentration 1mg/mL
Assay type Antibody validation/application
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:1
Rat
Mouse
ABMIUM Validated™ IHC
Species Dilution Notes

Specifications

Target
Cytokeratin 18
Also known as
Cell proliferation inducing gene 46 protein, CK 18, CYK 18, K 18, Keratin 18, Ketatin D, KRT18
Concentration
1mg/mL
Working concentration
WB 1:1,000-2,000 IHC 1:500-1,000
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WBIHC
Immunogen
Recombinant Protein
Observed band
46kDa
Cellular localization
Cytoplasm, Intermediate filament, Keratin, Nucleus
Dilution range
WB 1:1,000-2,000 IHC 1:500-1,000

Storage & Stability

Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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