Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM92152

Vinculin Rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

Vinculin Rabbit pAb from ELK Biotechnology Read more ›

Vinculin Rabbit pAb
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Vinculin Rabbit pAb

Product Description

Vinculin Rabbit pAb is a research-use antibody from ELK Biotechnology for studying Vinculin in WB, IHC workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Vinculin is a membrane-cytoskeletal protein in focal adhesion plaques that is involved in linkage of integrin adhesion molecules to the actin cytoskeleton. Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane.

Additional antibody information

Validation evidence captions Western blot analysis of 1) Hela, 2)K562, 3) Mouse Brain, 4)Rat Brain tissue with EA043 diluted at 1:1,000. | Immunohistochemical analysis of paraffin-embedded human Breast caricnoma using Vinculin (EA043)Rabbit pAb diluted at 1:500.

Key Facts

Product typePrimary Antibody Target: Vinculin
Collaborator ELK Biotechnology
Partner model EA043
Target Vinculin
Research areasAntibody Research
Reactivity Human,Rat,Mouse
Applications WB,IHC
ConjugationUnconjugated
Dilution rangeWB 1:1,000-2,000 IHC 1:200-500
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthetic Peptide
Concentration 1mg/mL
Assay type Antibody validation/application
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:1
Rat
Mouse
ABMIUM Validated™ IHC
Species Dilution Notes

Specifications

Target
Vinculin
Also known as
CMD1W antibody, CMH15 antibody, metavinculin antibody, MVCL antibody, VINC antibody
Concentration
1mg/mL
Working concentration
WB 1:1,000-2,000 IHC 1:200-500
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WBIHC
Immunogen
Synthetic Peptide
Observed band
130kDa
Cellular localization
Cytoplasm,Cytoskeleton,Cell junction, Adherens junction
Dilution range
WB 1:1,000-2,000 IHC 1:200-500

Storage & Stability

Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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