ABM92126
α-tubulin Rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
α-tubulin Rabbit pAb from ELK Biotechnology Read more ›
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Product Description
α-tubulin Rabbit pAb is an ELK Biotechnology antibody for research workflows involving α-tubulin.
Background
Tubulin is one of several members of a small family of globular proteins. The most common members of the tubulin family are α-tubulin and β-tubulin, the proteins that make up microtubules. Each has a molecular weight of approximately 55 kiloDaltons. Microtubules are assembled from dimers of α- and β-tubulin.
Additional antibody information
| Validation evidence captions | Western blot analysis of 1) Mouse skeletal muscle tissue, 2) Mouse kidney tissue, 3) Mouse Heart Tissue, 4) Mouse brain tissue, 5) Mouse Liver tissue with α-tubulin Rabbit pAb diluted at 1:2,000. | Western blot analysis of 1) Rat Brain tissue, 2) Rat Liver tissue, 3) Rat Heart Tissue, 4) HepG2, 5) 293T,with α-tubulin Rabbit pAb diluted at 1:2,000. | Western blot analysis of extracts from HeLa (Lane 1),MCF-7 (Lane 2), 3T3 (Lane 3), Rat Kidney (Lane 4), using α-Tubulin diluted at 1:3,000. |
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Key Facts
Product type
Primary Antibody›
Target: α-tubulin
| Target | α-tubulin |
| Concentration | 1mg/mL |
| Working concentration | WB 1:2,000 IHC 1:200 |
| Species reactivity | Human, Rat, Mouse |
| Observed band | 52kDa |
| Cellular localization | Cytoplasm,Cytoskeleton,Microtubule |
| Form / buffer | Antibody solution |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHC |
| Dilution range | WB 1:2,000 IHC 1:200 |
| Immunogen | Recombinant Protein |
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:2 | — |
| Rat | — | — |
| Mouse | — | — |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|
Specifications
Target
α-tubulin
Concentration
1mg/mL
Working concentration
WB 1:2,000 IHC 1:200
Form / buffer
Antibody solution
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human, Rat, Mouse
Tested applications
WBIHC
Immunogen
Recombinant Protein
Observed band
52kDa
Cellular localization
Cytoplasm,Cytoskeleton,Microtubule
Dilution range
WB 1:2,000 IHC 1:200
Storage & Stability
Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Storage conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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