β-actin Rabbit pAb
β-actin Rabbit pAb from ELK Biotechnology Read more ›
Product Description
β-actin Rabbit pAb is an ELK Biotechnology antibody for research workflows involving β-actin.
Background
β-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against β-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Actin may not be stable in certain cells. For example, expression of β-Actin in adipose tissue is very low and therefore β-Actin should not be used as loading control for these tissues.
Additional antibody information
| Validation evidence captions | Western blot analysis of HepG2 (1) , Rat liver (2) ,Mouse kidney (3) , Rabbit testic(4), Sheep lung(5), 293T(6) with β-Actin rabbit polyclonal antibody diluted at 1:5000. | IHC staining of Human ovary tissue with β-Actin pAb diluted at 1:200 | IF analysis of Hela with EA014 diluted at 1:500. |
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Key Facts
| Target | β-actin |
| Also known as | actin antibody, b-actin antibody, beta-actin antibody |
| Concentration | 1mg/mL |
| Working concentration | WB 1:3,000 IHC 1:200 IF 1:200 |
| Species reactivity | Human, Mouse, Rat, Rabbit, Chicken, Monkey, Sheep, Xenopus |
| Observed band | 43kDa |
| Cellular localization | Cytoplasm,Cytoskeleton |
| Form / buffer | Antibody solution |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHCIF |
| Dilution range | WB 1:3,000 IHC 1:200 IF 1:200 |
| Immunogen | Recombinant Protein |
Reactivity & Application Validation
8 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat | |||||||
| Rabbit | |||||||
| Chicken | |||||||
| Monkey | |||||||
| Sheep | |||||||
| Xenopus |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:3 | — |
| Mouse | — | — |
| Rat | — | — |
| Rabbit | — | — |
| Chicken | — | — |
| Monkey | — | — |
| Sheep | — | — |
| Xenopus | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.