β-actin Rabbit pAb from ELK Biotechnology Read more ›
β-actin Rabbit pAb is a research-use antibody from ELK Biotechnology for studying β-actin in WB, IHC, IF workflows with listed reactivity for Human, Mouse, Rat, Rabbit, Chicken, Monkey, Sheep, Xenopus. Specifications and supporting documents below should be reviewed when planning the experiment.
β-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against β-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Actin may not be stable in certain cells. For example, expression of β-Actin in adipose tissue is very low and therefore β-Actin should not be used as loading control for these tissues.
| Validation evidence captions | Western blot analysis of HepG2 (1) , Rat liver (2) ,Mouse kidney (3) , Rabbit testic(4), Sheep lung(5), 293T(6) with β-Actin rabbit polyclonal antibody diluted at 1:5000. | IHC staining of Human ovary tissue with β-Actin pAb diluted at 1:200 | IF analysis of Hela with EA014 diluted at 1:500. |
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| Collaborator | ELK Biotechnology |
| Partner model | EA014 |
| Target | β-actin |
| Research areas | Antibody Research |
| Reactivity | Human,Mouse,Rat,Rabbit,Chicken,Monkey,Sheep,Xenopus |
| Applications | WB,IHC,IF |
| Conjugation | Unconjugated |
| Dilution range | WB 1:3,000 IHC 1:200 IF 1:200 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant Protein |
| Concentration | 1mg/mL |
| Assay type | Antibody validation/application |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat | |||||||
| Rabbit | |||||||
| Chicken | |||||||
| Monkey | |||||||
| Sheep | |||||||
| Xenopus |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:3 | — |
| Mouse | — | — |
| Rat | — | — |
| Rabbit | — | — |
| Chicken | — | — |
| Monkey | — | — |
| Sheep | — | — |
| Xenopus | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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