His-Tag Rabbit pAb from ELK Biotechnology Read more ›
His-Tag Rabbit pAb is a research-use antibody from ELK Biotechnology for studying His-Tag in WB workflows with listed reactivity for Epitope tag. Specifications and supporting documents below should be reviewed when planning the experiment.
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells.
| Validation evidence captions | 1ug His fusion protein+ Primary antibody dilution at 1、1:5,000 2、1:10,000 | Primary antibody dilution at 1:5,000 1、293T transfeted with His tag recombinant protein 2、Baculovirus Expression Vector System transfected with His tag recombinant protein |
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| Collaborator | ELK Biotechnology |
| Partner model | EA004 |
| Target | His-Tag |
| Research areas | Antibody Research |
| Reactivity | Epitope tag |
| Applications | WB |
| Conjugation | Unconjugated |
| Dilution range | WB 1:5,000 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthetic Peptide |
| Concentration | 1mg/mL |
| Assay type | Antibody validation/application |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Epitope tag |
| Species | Dilution | Notes |
|---|---|---|
| Epitope tag | WB 1:5 | — |
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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