Calibrated Color Prestained Protein Marker (8-180kDa) is a protein marker from EnkiLife intended for research workflows. Review the listed specifications and supporting documents to confirm compatibility with the inte... Read more ›
Calibrated Color Prestained Protein Marker (8-180kDa) is a protein marker from EnkiLife intended for research workflows. Review the listed specifications and supporting documents to confirm compatibility with the intended method.
This product is a high-purity recombinant color prestained protein marker specifically designed for research experiments. Through scientific proportioning of 10 precise molecular weight proteins (8, 16.5, 25, 31, 41, 52, 72, 95, 130, 180kDa), it addresses the core pain points of traditional prestained markers "low accuracy and poor stability" providing key support for improved experimental efficiency:
Color differentiation design: 72kDa (orange-red), 8kDa (green), others (blue), allowing real-time observation without staining, no need to pause electrophoresis to judge progress, quick confirmation of transfer efficiency after membrane transfer
Authoritative calibration guarantee: Calibrated with imported brand non-prestained marker standards, molecular weight deviation is far below industry average, accuracy comparable to non-prestained markers
Pre-mixed 1× SDS-PAGE loading buffer, no boiling required
Broad molecular weight coverage: 8-180kDa covers commonly used protein molecular weight ranges in research
Clear band performance: 3-5μL loading volume produces sharp bands, no diffusion or trailing after electrophoresis
| Product Name | Specification 1 (100μL) | Specification 2 (250μL×2) | Specification 3 (250μL×10) |
|---|---|---|---|
| Calibrated Color Prestained Protein Marker (8-180kDa) | Suitable for small batch pre-experiments (approx. 20-30 uses) | Suitable for regular laboratories (approx. 100-150 uses) | Suitable for large laboratories/high-throughput experiments (approx. 500-800 uses) |
1. Molecular Weight Accuracy: Comprehensive Leadership Over Domestic and International Counterparts
Comparison with international mainstream brands (key molecular weight node deviations referenced to 26614):
| Molecular Weight Node | This Product Deviation | International T Brand Deviation |
|---|---|---|
| 180kDa | ＜2% | 17.2%（180→149kDa） |
| 95kDa&100kDa | ＜2% | 15%（100→85kDa） |
| 72kDa&70kDa | 9.7%（72→65kDa） | 7.1%（70→65kDa） |
| 52kDa&55kDa | ＜2%（52kDa） | 9.1%（55→50kDa） |
| 31kDa&35kDa | ＜2%（31kDa） | 17.1%（35→29kDa） |
2. Buffer System Compatibility: Stable Migration Across Scenarios
Validated across multiple systems including Tris-Glycine buffer (conventional SDS-PAGE system), Tris-Tricine buffer (small molecule protein separation system), and Bis-Tris buffer (high-resolution separation system), this product maintains stable migration in all systems
3. Extreme Condition Stability: Handling Complex Experimental Environments
Repeated freeze-thaw testing: 50 freeze-thaw cycles (-20℃→room temperature), no significant changes in band clarity or molecular weight migration, meeting laboratory scenarios requiring "frequent access"
High temperature stability testing: 7 days at 40℃ simulating transportation environment, no protein degradation or aggregation, band integrity maintained well, addressing "summer transportation deterioration" concerns
Room temperature storage testing: 30 days at 25℃ room temperature, no observed band changes, short-term forgetting to return to refrigerator does not affect use
4. Operational Convenience: Cost-Reducing, Efficiency-Enhancing Research Assistant
Precautions
This product already contains loading buffer, do not heat or boil;
To reduce marker degradation and contamination risks, recommend aliquoting and storing at -20℃;
For research use only.
| Collaborator | EnkiLife |
| Applications | WB |
| Shelf life | 2 years from manufacture |
| Availability | In stock - ships within 3 business days |
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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